asc1 gfp mice Search Results


asc1 gfp mice  (Jackson Laboratory)
86
Jackson Laboratory asc1 gfp mice
Asc1 Gfp Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asc1+gfp+mice/bio_rxiv__2025__08__06__668799-238-0-6?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
asc1 gfp mice - by Bioz Stars, 2026-07
86/100 stars
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mouse anti mcherry  (Abcam)
99
Abcam mouse anti mcherry
( a ) Schematic representation of the domain organization of fluorophore-tagged <t>(mCherry</t> or enhanced GFP (eGFP)) WT ASC (ASC FL ), ASC PYD and ASC CARD constructs. ( b ) Release of LDH (assessing cell death) <t>and</t> <t>IL-1β</t> from LPS-primed immortalized WT, Asc −/− or Asc −/− BMDMs expressing ASC FL , ASC PYD or ASC CARD 3 h after poly(dA:dT) transfection (1 μg ml −1 ). ( c ) Representative images of cell lines from ( b ) 3 h after poly(dA:dT) transfection (1 μg ml −1 ) or 1 h after ATP treatment (5 mM). DNA (blue, Hoechst), ASC FL or ASC PYD (red) and ASC CARD (green). Scale bars, 10 μm. Data ( b , c ) are representative of three independent experiments. Graphs show the mean and s.d. from quadruplicate wells. See also .
Mouse Anti Mcherry, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asc1+gfp+mice/pmc04917984-178-53-56?v=Abcam
Average 99 stars, based on 1 article reviews
mouse anti mcherry - by Bioz Stars, 2026-07
99/100 stars
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Image Search Results


( a ) Schematic representation of the domain organization of fluorophore-tagged (mCherry or enhanced GFP (eGFP)) WT ASC (ASC FL ), ASC PYD and ASC CARD constructs. ( b ) Release of LDH (assessing cell death) and IL-1β from LPS-primed immortalized WT, Asc −/− or Asc −/− BMDMs expressing ASC FL , ASC PYD or ASC CARD 3 h after poly(dA:dT) transfection (1 μg ml −1 ). ( c ) Representative images of cell lines from ( b ) 3 h after poly(dA:dT) transfection (1 μg ml −1 ) or 1 h after ATP treatment (5 mM). DNA (blue, Hoechst), ASC FL or ASC PYD (red) and ASC CARD (green). Scale bars, 10 μm. Data ( b , c ) are representative of three independent experiments. Graphs show the mean and s.d. from quadruplicate wells. See also .

Journal: Nature Communications

Article Title: ASC filament formation serves as a signal amplification mechanism for inflammasomes

doi: 10.1038/ncomms11929

Figure Lengend Snippet: ( a ) Schematic representation of the domain organization of fluorophore-tagged (mCherry or enhanced GFP (eGFP)) WT ASC (ASC FL ), ASC PYD and ASC CARD constructs. ( b ) Release of LDH (assessing cell death) and IL-1β from LPS-primed immortalized WT, Asc −/− or Asc −/− BMDMs expressing ASC FL , ASC PYD or ASC CARD 3 h after poly(dA:dT) transfection (1 μg ml −1 ). ( c ) Representative images of cell lines from ( b ) 3 h after poly(dA:dT) transfection (1 μg ml −1 ) or 1 h after ATP treatment (5 mM). DNA (blue, Hoechst), ASC FL or ASC PYD (red) and ASC CARD (green). Scale bars, 10 μm. Data ( b , c ) are representative of three independent experiments. Graphs show the mean and s.d. from quadruplicate wells. See also .

Article Snippet: The following antibodies and dilutions were used: rat anti-Caspase-1 p20 (Genentech, 1:1,000; ) or mouse anti-Caspase 1 p20 (1:4,000, AG20B-0042, AdipoGen; and ), rat anti-ASC (Genentech, 1:2,000; and ) or rabbit anti-ASC (1:1,000, AG25B-006, Adipogen, all other figures), rabbit anti-IL-18 (5180R-100, Biovision, 1:1,000), goat anti-IL-1β (AF-401-NA, R&D, 1:1,000), mouse anti-GFP (632381, Clontech, 1:1,000), mouse anti-mCherry (ab125096, Abcam, 1:1,000), mouse anti-V5 (R960-25, Invitrogen, 1:1,000), rabbit anti-HMGB1 (GTX-101277, Genetex, 1:1,000), mouse anti-gasdermin-D (GSDMDC1 (A-7), Santa Cruz Biotechnology, 1:1,000; ), rabbit anti-gasdermin-D (1:2,000, G7422, Sigma, all other figures) and mouse anti-β-actin (A1987, Sigma, 1:1,000).

Techniques: Construct, Expressing, Transfection

( a ) Structural model of the mouse ASC CARD based on the human homologue (PDB 2KN6 (ref. )). The structure is shown in ribbon (left) and electrostatic surface representation (right, blue, positive charge; red, negative charge). Residues D130 and D134, involved in the interaction with pro-caspase-1, are highlighted . ( b ) Release of LDH and IL-1β from LPS-primed immortalized Asc −/− BMDMs and Asc −/− BMDMs expressing ASC FL , ASC D130R or ASC D134R 3 h after poly(dA:dT) transfection (1 μg ml −1 ). ( c , d ) Representative images of cell lines from ( b ) 3 h after poly(dA:dT) transfection (1 μg ml −1 ) ( c ) or 1 h after ATP treatment (5 mM) ( d ). DNA was stained with Hoechst (blue) and ASC (red). Scale bars, 10 μm. ( e , f ) Measurement of the ASC speck diameter in primary C57BL/6 BMDMs transduced with the indicated ratio of mCherry-tagged ASC FL or ASC D130R and transfected with poly(dA:dT) (3 h at 1 μg ml −1 ( e )) or treated with nigericin (1 h, 20 μM ( f )) after LPS priming. ( g ) Representative images from e . DNA was stained with Hoechst (blue) and ASC (red). Scale bars, 5 μm. Data are representative of three ( b – d ) independent experiments. Graphs show the mean and s.d. from quadruplicate wells ( b ) or triplicate coverslips ( e , f ). The numbers of specks measured were 99, 92, 104, 94 and 108 in ( e ) and 149, 134, 85, 98 and 95 in ( f ). * P <0.05, ** P <0.01 and *** P <0.001 (one-way analysis of variance). See also .

Journal: Nature Communications

Article Title: ASC filament formation serves as a signal amplification mechanism for inflammasomes

doi: 10.1038/ncomms11929

Figure Lengend Snippet: ( a ) Structural model of the mouse ASC CARD based on the human homologue (PDB 2KN6 (ref. )). The structure is shown in ribbon (left) and electrostatic surface representation (right, blue, positive charge; red, negative charge). Residues D130 and D134, involved in the interaction with pro-caspase-1, are highlighted . ( b ) Release of LDH and IL-1β from LPS-primed immortalized Asc −/− BMDMs and Asc −/− BMDMs expressing ASC FL , ASC D130R or ASC D134R 3 h after poly(dA:dT) transfection (1 μg ml −1 ). ( c , d ) Representative images of cell lines from ( b ) 3 h after poly(dA:dT) transfection (1 μg ml −1 ) ( c ) or 1 h after ATP treatment (5 mM) ( d ). DNA was stained with Hoechst (blue) and ASC (red). Scale bars, 10 μm. ( e , f ) Measurement of the ASC speck diameter in primary C57BL/6 BMDMs transduced with the indicated ratio of mCherry-tagged ASC FL or ASC D130R and transfected with poly(dA:dT) (3 h at 1 μg ml −1 ( e )) or treated with nigericin (1 h, 20 μM ( f )) after LPS priming. ( g ) Representative images from e . DNA was stained with Hoechst (blue) and ASC (red). Scale bars, 5 μm. Data are representative of three ( b – d ) independent experiments. Graphs show the mean and s.d. from quadruplicate wells ( b ) or triplicate coverslips ( e , f ). The numbers of specks measured were 99, 92, 104, 94 and 108 in ( e ) and 149, 134, 85, 98 and 95 in ( f ). * P <0.05, ** P <0.01 and *** P <0.001 (one-way analysis of variance). See also .

Article Snippet: The following antibodies and dilutions were used: rat anti-Caspase-1 p20 (Genentech, 1:1,000; ) or mouse anti-Caspase 1 p20 (1:4,000, AG20B-0042, AdipoGen; and ), rat anti-ASC (Genentech, 1:2,000; and ) or rabbit anti-ASC (1:1,000, AG25B-006, Adipogen, all other figures), rabbit anti-IL-18 (5180R-100, Biovision, 1:1,000), goat anti-IL-1β (AF-401-NA, R&D, 1:1,000), mouse anti-GFP (632381, Clontech, 1:1,000), mouse anti-mCherry (ab125096, Abcam, 1:1,000), mouse anti-V5 (R960-25, Invitrogen, 1:1,000), rabbit anti-HMGB1 (GTX-101277, Genetex, 1:1,000), mouse anti-gasdermin-D (GSDMDC1 (A-7), Santa Cruz Biotechnology, 1:1,000; ), rabbit anti-gasdermin-D (1:2,000, G7422, Sigma, all other figures) and mouse anti-β-actin (A1987, Sigma, 1:1,000).

Techniques: Expressing, Transfection, Staining, Transduction

( a ) Release of LDH and IL-1β from LPS-primed immortalized WT, Asc −/− or Asc −/− BMDMs expressing ASC FL , ASC PYD or ASC CARD after infection with log-phase WT S. Typhimurium SL1344 (multiplicity of infection (MOI) 10 for 1 h). ( b ) Quantification of the percentage of cells from a with ASC specks or filaments (collectively referred to as ASC aggregates). ( c ) Representative images of cell lines from b . DNA (blue, Hoechst) and ASC (red). Scale bars, 10 μm. ( d ) Release of LDH and IL-1β from LPS-primed Asc −/− BMDMs and Asc −/− BMDMs expressing ASC FL , ASC D130R or ASC D134R after infection with log-phase WT S. Typhimurium SL1344 (MOI 10 for 1 h). ( e ) Quantification of the percentage of cells with ASC aggregates from d . ( f ) Measurement of the ASC speck diameter in primary C57BL/6 BMDMs transduced with the indicated ratio of mCherry-tagged ASC FL or ASC D130R and infected with log-phase WT S. Typhimurium SL1344 (MOI 10 for 1 h) after LPS priming. Data are representative of three ( a – e ) independent experiments. Graphs show the mean and s.d. from quadruplicate wells in ( a , c – e ) and triplicate coverslips in f . The numbers of specks measured were 194, 134, 184, 128 and 141 in f . * P <0.05, ** P <0.01 and *** P <0.001 (one-way analysis of variance). See also .

Journal: Nature Communications

Article Title: ASC filament formation serves as a signal amplification mechanism for inflammasomes

doi: 10.1038/ncomms11929

Figure Lengend Snippet: ( a ) Release of LDH and IL-1β from LPS-primed immortalized WT, Asc −/− or Asc −/− BMDMs expressing ASC FL , ASC PYD or ASC CARD after infection with log-phase WT S. Typhimurium SL1344 (multiplicity of infection (MOI) 10 for 1 h). ( b ) Quantification of the percentage of cells from a with ASC specks or filaments (collectively referred to as ASC aggregates). ( c ) Representative images of cell lines from b . DNA (blue, Hoechst) and ASC (red). Scale bars, 10 μm. ( d ) Release of LDH and IL-1β from LPS-primed Asc −/− BMDMs and Asc −/− BMDMs expressing ASC FL , ASC D130R or ASC D134R after infection with log-phase WT S. Typhimurium SL1344 (MOI 10 for 1 h). ( e ) Quantification of the percentage of cells with ASC aggregates from d . ( f ) Measurement of the ASC speck diameter in primary C57BL/6 BMDMs transduced with the indicated ratio of mCherry-tagged ASC FL or ASC D130R and infected with log-phase WT S. Typhimurium SL1344 (MOI 10 for 1 h) after LPS priming. Data are representative of three ( a – e ) independent experiments. Graphs show the mean and s.d. from quadruplicate wells in ( a , c – e ) and triplicate coverslips in f . The numbers of specks measured were 194, 134, 184, 128 and 141 in f . * P <0.05, ** P <0.01 and *** P <0.001 (one-way analysis of variance). See also .

Article Snippet: The following antibodies and dilutions were used: rat anti-Caspase-1 p20 (Genentech, 1:1,000; ) or mouse anti-Caspase 1 p20 (1:4,000, AG20B-0042, AdipoGen; and ), rat anti-ASC (Genentech, 1:2,000; and ) or rabbit anti-ASC (1:1,000, AG25B-006, Adipogen, all other figures), rabbit anti-IL-18 (5180R-100, Biovision, 1:1,000), goat anti-IL-1β (AF-401-NA, R&D, 1:1,000), mouse anti-GFP (632381, Clontech, 1:1,000), mouse anti-mCherry (ab125096, Abcam, 1:1,000), mouse anti-V5 (R960-25, Invitrogen, 1:1,000), rabbit anti-HMGB1 (GTX-101277, Genetex, 1:1,000), mouse anti-gasdermin-D (GSDMDC1 (A-7), Santa Cruz Biotechnology, 1:1,000; ), rabbit anti-gasdermin-D (1:2,000, G7422, Sigma, all other figures) and mouse anti-β-actin (A1987, Sigma, 1:1,000).

Techniques: Expressing, Infection, Transduction